Abacus Diagnostics – Dramatically Improving Crime Scene Investigation

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Compare the strengths & weaknesses of various technologies

Semen Identification Test for the forensic and crime scene use

	Sensitivity	Specificity	Storage	Running Buffer Required	Extraction Buffer Required	Extraction Time(incl reagent prep)	Minimum Sensitivity	Labor Intensive/ Extensive Prep required
ABAcard® p30	Ref	Ref	Room Temp	No	Yes	Ref	4 ng/ml	No
Semenogelin	Ref 1 Ref 2 Ref 3 Ref 4 Ref 5	Ref 1 Ref 2 Ref3 Ref4 Ref5 Ref 6 Ref 7 Ref 8	N/A	N/A	Yes	N/A	N/A	N/A
Cross Over Electrophoresis	N/A	N/A	Refrigeration	N/A	Yes	N/A	4000 ng/ml	Yes Multiple Step, Longest

Blood Identification Test for the forensic and crime scene use

	Minimum Sensitivity	Specificity	Storage	Running Buffer Required	Extraction Buffer Required	Extraction Time	Minimum Sensitivity	Labor Intensive/ Extensive Prep required
Hematrace^®	Ref	Ref	Room Temp	No	Yes	Ref	0.05 µg/ml	No
Glycophorin	N/A	Ref1 Ref2 Ref3	N/A	N/A	N/A	1-2 hours	No (1 µl of blood)	N/A
Hemastix	0.001 µl of blood	N/A	Room Temp	No	No	1 minute	No	No

Saliva Identification Test for the forensic and crime scene use

SALIgAE^® Protocol Presidents DNA Initiative National Institute of Justice, U.S. Department of Justice

	Sensitivity	Specificity	Buffer Required	Extraction Time	Ease of Use Visible result	Labor Intensive/Extensive Prep required
SALIgAE ®	Ref	Ref	Ref	Ref	Color Change	No
Salivary Amylase Antibody	Ref	Ref Ref	Yes	N/A	N/A	Yes
Radial Gel Diffusion	Medium	High	Yes, Multiple ones Needs Refrigeration	30 minutes	cumbersome	Yes
Liquid Phase Amylase Test	Highest Ref	Lowest Ref	Yes, Multiple ones	30 minutes	cumbersome	Yes